Study on the application of model transfer technology in the extraction process of Xiao'er Xiaoji Zhike oral liquid / 药学学报

The modernization and development of traditional Chinese medicine has led to higher standards for the quality of traditional Chinese medicine products. The extraction process is a crucial component of traditional Chinese medicine production, and it directly impacts the final quality of the product. However, the currently relied upon methods for quality assurance of the extraction process, such as simple wet chemical analysis, have several limitations, including time consumption and labor intensity, and do not offer precise control of the extraction process. As a result, there is significant value in incorporating near-infrared spectroscopy (NIRS) in the production process of traditional Chinese medicine to improve the quality control of the final products. In this study, we focused on the extraction process of Xiao'er Xiaoji Zhike oral liquid (XXZOL), using near-infrared spectra collected by both a Fourier transform near-infrared spectrometer and a portable near-infrared spectrometer. We used the concentration of synephrine, a quality control index component specified by the pharmacopoeia, to achieve rapid and accurate detection in the extraction process. Moreover, we developed a model transfer method to facilitate the transfer of models between the two types of near-infrared spectrometers (analytical grade and portable), thus resolving the low resolution, poor performance, and insufficient prediction accuracy issues of portable instruments. Our findings enable the rapid screening and quality analysis of XXZOL onsite, which is significant for quality monitoring during the traditional Chinese medicine production process.

Effect of Bailing capsule on the patients with stable chronic obstructive pulmonary disease / 新乡医学院学报

Objective To investigate the effect and mechanism of Bailing capsule on the patients with stable chronic obstructive pulmonary disease (COPD).Methods A total of 100 patients with more than two weeks of stable COPD were selected from January 2013 to June 2016 in the Department of Respiratory Medicine,the First Affiliated Hospital of Xinxiang Medical University.The patients were randomly divided into the control group and the observation group,with 50 cases in each group.The patients in the control group were treated with tiotropium bromide powder for inhalation,and the patients in the observation group were treated with Bailing capsule orally on the basis of tiotropium bromide powder for inhalation,the patients in the two groups were treated for 12 months.The clinical effect and the average number of acute exacerbations within one year were compared between the two groups;and the level of serum amyloid A (SAA) were compared between the two groups before treatment and 3,6 and 12 months after treatment.Results The total effective rate in the observation group (84.0％) was significantly higher than that in the control group (72.0％)after 12 months of treatment (x2 =4.097,P ＜ 0.05).The average number of acute exacerbations in the observation group within one year was significantly less than that in the control group (P ＜ 0.01).There was no significant difference in serum SAA level between the two groups before treatment and 3 months after treatment (P ＞ 0.05).The serum SAA level in the observation group was significantly lower than that in the control group at the time points of 6 and 12 months after treatment (P ＜ 0.05).There was no significant difference in serum SAA level between before treatment and 3 months after treatment in the two groups (P ＞ 0.05).The serum SAA level at 6 and 12 months after treatment was significantly lower than that before treatment and 3 months after treatment in the two groups (P ＜ 0.05).The serum SAA level at 12 months of treatment was significantly lower than that at 6 months of treatment in the two groups (P ＜ 0.05).Conclusion Bailing capsule can inhibit the inflammatory reaction of COPD patients by interfering with the secretion of serum SAA,so as to improving the clinical manifestations of COPD patients and reducing the acute attack of COPD.

Intervention effect of dihydroartemisinin on pulmonary fibrosis in rats / 中华实用儿科临床杂志

Objective To investigate the effect of dihydroartemisinin on pulmonary fibrosis and its mechanism in rats.Methods Seventy-five SD rats were randomly divided into 5 groups:the control group,the model group,the high dihydroartemisinin group,the low dihydroartemisinin group,and the prednisone group(15 rats in each group).Saline(9 g/L,0.3 mL) was injected into the rat trachea of the control group,and the rats of the rest 4 groups were injected bleomycin(3 mg/kg).The rats in the control group and the model group received saline lavage (9 g/L,3 mL),while the rats in the high dihydroartemi-sinin group and the low dihydroartemisinin group received dihydroartemisinin lavage (100,50 mg/kg),and the rats in the prednisone group received prednisone lavage each day.Five rats in each group were sacrificed and lung tissues were removed on the 8th,15th,and 29th day of lavage,and the degree of inflammation in lung tissue was observed by HE staining.The deposit of collagen in lung tissue was observed by Masson staining.The expressions of transforming growth factor β1 (TGF-β1),collagen Ⅰ and collagen Ⅲ in pneumonic tissues were detected by immunohistochemistry.Results The degree of inflammation in pneumonic tissues in the high dihydroartemisinin group,the low dihydroartemisinin group,and the prednisone group were less significantly than that in the model group(all P ＜ 0.01).The deposit of collagen in lung tissues in the model group was obviously increased than that of the control group at each time points(all P ＜0.01).The deposit of collagen in lung tissues in the high dihydroartemisinin group,the low dihydroartemisinin group and the prednisone group were lower than that in the model group(all P ＜0.01),and higher than that of the control group at each time points(all P ＜0.01).There was no significant difference between the high dihydroartemisinin group and the prednisone group (P ＞ 0.05).TGF-β1,collagen Ⅰ and collagen Ⅲ in lung tissues in the high dihydroartemisinin group and the prednisone group were lower than those in the model group(P ＜0.01),and higher than those in the control group at each time points(all P ＜ 0.01).There was no significant difference in TGF-β1,collagen Ⅰ and collagen Ⅲ in lung tissue between the high dihydroartemisinin group and the prednisone group(all P ＞0.05),except that collagen Ⅲ expression in lung tissues was lower than that of the prednisone group on the 29th day (P ＞ 0.05).Conclusions Dihydroartemisinin can lessen the extent of pulmonary fibrosis in rats and its anti-fibrosis effect is dose-dependent.The anti-fibrosis effect of dihydroartemisinin may decrease the deposit of collagens in the lung tissues by inhibiting TGF-β1 expression in the lung tissues and have an effect of antifibrosis.

Effects of hot shock protein 70 inhibitor PFTµ on inflammatory response in lipopolysaccharide-stimulated RAW264.7 cells and mice underwent myocardial ischemia-reperfusion injury / 中华心血管病杂志

<p><b>OBJECTIVE</b>To observe the effects of hot shock protein 70 (HSP70) inhibitor (PFTµ) on inflammation response in lipopolysaccharide (LPS)-stimulated RAW264.7 cells and mice underwent myocardial ischemia-reperfusion (I/R) injury.</p><p><b>METHODS</b>RAW264.7 macrophage line of mice was stimulated by LPS as an inflammatory model. These were divided into control (15 min DMSO pretreatment and LPS 2 g/L) and PFTµ treated groups (15 min PFTµ 20 µmol/L pretreatment and LPS 2 g/L). NO concentration was measured by Griess Kit. The expression of iNOS protein and mRNA were detected by Western blot and RT-PCR. Infarct size was determined on mice underwent myocardial ischemia-reperfusion (I/R) injury in the absence or presence (PFTµ 40 mg/kg, intraperitoneal injection).</p><p><b>RESULTS</b>PFTµ significantly blocked the production of NO and protein and mRNA expression of iNOS (P < 0.05 vs. control). PFTµ also significantly reduced the infarct size on mice underwent I/R injury (P < 0.05 vs. control).</p><p><b>CONCLUSION</b>These results suggest that PFTµ could be a potential therapeutic agent for the treatment of inflammatory diseases through inhibiting the production of NO and reducing inflammatory responses.</p>

Significance of serum cytokines concentrations and APACHE scores in evaluating the illness state for Critical patients / 中国基层医药

Objective To investigate the clinical significance of serum cytokines concentrations and A- PACHE scores in evaluating the illness state for critical trauma patients.Methods A clinical prospective self-control trial was performed,in which 36 patients admitted to ICU by SIRS were enrolled.Objects were divided into mild and severe trauma group according to APACHE score.The TNF-?and IL-6 concentrations were determined on the 1st, 3rd and 5th day of admission,the APACHE score were assessed at the same time.Statistic analysis was performed according to this group.Results The TNF-?concentrations decreased continuously in the following days while IL-6 decreased from the 7th day in the mild trauma group.In the severe trauma group the TNF-?and IL-6,APACHE score concentrations kept increasing.There was a significant difference of TNF-?and IL-6 concentrations between severe trauma and mild trauma group.Conclusion Dynamic measurement of TNF-?and IL-6 concentrations with APACHE score provide great help to evaluate the illness state and predict the prognosis.